high-performance liquid chromatography method for determination of 1-(2-phenylethyl)-5-(quinaldin-4-yl) biuret (a potential cytotoxic agent) in rat plasma

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development and validation of a high-performance liquid chromatography method with uv detection for determination of 1-(2-phenylethyl)-5- (quinaldin-4-yl) biuret in rat plasma

results under the above chromatography condition, biuret compound (peqb) and the internal standard were detected at 4.5 and 13.5 minutes, respectively. limit of quantitation of the peqb was 0.1 µg/ml. accuracy of the method over the concentration range of 0.1-100 µg/ml was between 88-109%. inter- and intraday precisions were 4-19% and 6-8%, respectively. a good relationship in the form of a pow...

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Development and Validation of a High-Performance Liquid Chromatography Method With UV Detection for Determination of 1-(2-phenylethyl)-5- (quinaldin-4-yl) Biuret in Rat Plasma

BACKGROUND Recently, biuret derivatives have been reported as showing moderate to good cytotoxic effect against certain cancer cell lines. In this study, a high-performance liquid chromatography method was developed for determination of 1-(2-phenylethyl)-5-(quinaldin-4-yl) biuret (PEQB) in rat plasma to use in future studies on this compound and related derivatives. OBJECTIVES In this study, ...

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High-Performance Liquid Chromatography Determination of Methotrexate in Plasma

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A simple, efficient, accurate and selective HPLC method has been developed and validated successfully for the estimation of 5-fluorouracil in rabbit plasma. The drug was eluted by using Supelco C18 column (1.5 cm x 4.6 mm, 5 μm) with a mobile phase consisting of methanol and water (20: 80, v/v) by adjusting pH at 3.2, using perchloric acid solution. The retention time was found to be 4.107 with...

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عنوان ژورنال:
research in pharmaceutical sciences

جلد ۷، شماره ۵، صفحات ۰-۰

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